pher4  (WuXi AppTec)

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues

doi: 10.1369/jhc.2008.952911

Figure Lengend Snippet: Antiphosphoprotein antibodies

Article Snippet: The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml pHER4 Abgent (San Diego, CA) Y1 162 Rabbit 0.62 pg/ml pAkt Cell Signaling S473 Rabbit 0.1 pg/ml pMAPK Cell Signaling T202/Y204 Rabbit 0.5 ng/ml pTyr R&D pan T Mouse 2 pg/ml pp53 Abcam (Cambridge, MA) S315 Rabbit 0.2 pg/ml Open in a separate window Antiphosphoprotein antibodies To confirm the specificity of phosphoprotein staining, lambda phosphatase was applied on the sections before incubation with primary antibodies.

Techniques: Concentration Assay

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues

doi: 10.1369/jhc.2008.952911

Figure Lengend Snippet: IHC staining of phosphoproteins in xenograft tumor tissues

Article Snippet: The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml pHER4 Abgent (San Diego, CA) Y1 162 Rabbit 0.62 pg/ml pAkt Cell Signaling S473 Rabbit 0.1 pg/ml pMAPK Cell Signaling T202/Y204 Rabbit 0.5 ng/ml pTyr R&D pan T Mouse 2 pg/ml pp53 Abcam (Cambridge, MA) S315 Rabbit 0.2 pg/ml Open in a separate window Antiphosphoprotein antibodies To confirm the specificity of phosphoprotein staining, lambda phosphatase was applied on the sections before incubation with primary antibodies.

Techniques: Immunohistochemistry

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues

doi: 10.1369/jhc.2008.952911

Figure Lengend Snippet: IHC staining of phosphoproteins in xenograft and human clinical tumor tissues. (A) SKOV-3 xenograft tumor tissues fixed in Streck’s tissue fixative (STF), 10% formalin, and 4% paraformaldehyde (PFA) were stained with anti-pMet antibody. (B) Human lung tumor tissue fixed in 10% formalin and STF were stained with anti-pHER4 antibody. Bar = 50 μm.

Article Snippet: The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml pHER4 Abgent (San Diego, CA) Y1 162 Rabbit 0.62 pg/ml pAkt Cell Signaling S473 Rabbit 0.1 pg/ml pMAPK Cell Signaling T202/Y204 Rabbit 0.5 ng/ml pTyr R&D pan T Mouse 2 pg/ml pp53 Abcam (Cambridge, MA) S315 Rabbit 0.2 pg/ml Open in a separate window Antiphosphoprotein antibodies To confirm the specificity of phosphoprotein staining, lambda phosphatase was applied on the sections before incubation with primary antibodies.

Techniques: Immunohistochemistry, Staining

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues

doi: 10.1369/jhc.2008.952911

Figure Lengend Snippet: IHC staining of phosphoproteins in human clinical tumor tissues

Article Snippet: The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml pHER4 Abgent (San Diego, CA) Y1 162 Rabbit 0.62 pg/ml pAkt Cell Signaling S473 Rabbit 0.1 pg/ml pMAPK Cell Signaling T202/Y204 Rabbit 0.5 ng/ml pTyr R&D pan T Mouse 2 pg/ml pp53 Abcam (Cambridge, MA) S315 Rabbit 0.2 pg/ml Open in a separate window Antiphosphoprotein antibodies To confirm the specificity of phosphoprotein staining, lambda phosphatase was applied on the sections before incubation with primary antibodies.

Techniques: Immunohistochemistry